On our way to sub-second separations of enantiomers in high-performance liquid chromatography

Author: nana khundadze
Co-authors: Salome Pantsulaia, Rusudan Kakava
Keywords: Fast separation of enantiomers. Segmented chiral-achiral columns. Enantiomers. Separation. Column
Annotation:

In recent years, intensive study of superficially porous silica is used for achiral separation. The core of such silica is nonporous, but the surface is porous. The advantage of such morphology is that the surface area of the porous shell is sufficient for effective chromatographic separations. The rational of this concept was that columns packed with such particles would have a higher efficiency, because diffusion through the thin porous layer surrounding the particles would be faster than the diffusion through the whole particles. This fact accelerates mass transfer between mobile and stationary phases and sharply improves the chromatographic separation efficiency. Chiral absorbents prepared on the basis of superficially porous silica have wide range of advantages – small amount of dead volume, a high number of theoretical plates, the slow decrease in the theoretical plates in the high flow rates of mobile phase, because these types of columns are packed with homogenous particles , it reduces band broadening. As a result of preliminary research done within the undergraduate work, it has been confirmed that it is possible to get enantiomer separation in a few seconds ( 1.5 in a separate case). Main goal of conducting the previous experiment is finding the fastest way to separate the enantiomers. The material obtained in the experiment suggests that it is possible to separate the enantiomer within a period of less than 1 second by optimizing the conditions of the analytical substances, the content of the chiral selector, the column length and the chromatographic separation conditions.